Macau is a “first-to-file” jurisdiction, which means that, as a general rule, you only obtain rights over your trademark if you register it. Unregistered marks may ser protected only in very exceptional situations, so relying on use alone is risky.
Although Macau is a Special Administrative Region of China, it has an independent trademark system. A trademark registered in China does not grant protection in Macau. To secure protection, you must file directly with the Intellectual Property Office of Macau.
It is also worth noting that use of the mark is not required in order to file or obtain registration in Macau, so you can and should register even before starting commercial use.